Analysis of the genitor origin of an intergeneric hybrid clone between Zea and Tripsacum for forage production by McGISH.

作者:

Yan Xu,Li Yingzheng,Wu Zizhou,Li Yang,Wen Xiaodong,Li Xiaofeng,He Ruyu,Yang Chunyan,Zhao Yanli,Cheng Mingjun,Zhang Ping,Sam Ebenezer Kofi,Rong Tingzhao,He Jianmei,Tang Qilin

作者背景:

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Animal Husbandry Research Center & Sericulture Research Institute, Sichuan Academy of Agricultural Sciences, No. 52 Hezhong Street Shunqing District, Nanchong, 637000, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Guizhou Prataculture Institute, No. 1 Jinnong Road Huaxi District, Guiyang, 550006, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Sichuan Grass Industry Technology Research and Promotion Center, No. 4 Wuhouci Street, Chengdu, 610041, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

· Maize Research Institute, Sichuan Agricultural University, No. 211 Huimin Road Wenjiang District, Chengdu, 611130, China P.R.

DOI:10.1270/jsbbs.19107

文章关键词:McGISH,Tripsacum,Zea,Forage breeding,Perennial forage maize

原文摘要:In this study, the chromosome number and composition of a novel perennial forage crop, 'Yucao No. 6' (Yu6), was revealed by chromosome spread and McGISH (multicolor genomic in situ hybridization) techniques to clarify its genitor origin. Cytogenetic analysis showed that Yu6, which has 56 chromosomes, is an aneuploid representing 12, 17 and 27 chromosomes from Zea mays ssp. mays L. (Zm, 2n = 2x = 20), Tripsacum dactyloides L. (Td, 2n = 4x = 72), and Z. perennis (Hitchc.) Reeves & Mangelsd. (Zp, 2n = 4x = 40), respectively. This finding indicates that Yu6 is the product of a reduced egg (n = 36 = 12Zm + 17Td + 7Zp) of MTP (a near-allohexaploid hybrid, 2n = 74 = 20Zm + 34Td + 20Zp) fertilized by a haploid sperm nucleus (n = 20Zp) of Z. perennis . Moreover, 3 translocated chromosomes consisting of the maize-genome chromosome with the segment of Z. perennis were observed. These results suggest that it is practical to develop perennial forage maize by remodeling the chromosomal architecture of MTP offspring with Z. perennis as a pollen parent. Finally, the overview of forage breeding in the Zea and Tripsacum genera was discussed.