Improving Inorganic Phosphorus Content in Maize Seeds by Introduction of Phytase Gene

Journal: Biotechnology.2008,7: 323-327

Author: Yaou Shen, Hongning Wang, Guangtang Pan

Abstract:

In our research, the plant endosperm special expression vector pCBA with target gene phyA was introduced into maize inbred line 18-599 (red) by microprojectile bombardment transformation. The result of PCR assay and Southern hybridization for regenerated plants showed genomes of seven plants were embedded with target gene phyA. Of the seven plants, seeds of four plants were collected successfully. The result of phytase activity assay revealed, phytase activities of dry seeds of three plants exhibited distinct increase compared to the control. The top phytase activity of them increased by 60.85% and the according inorganic phosphate content increased by 47.84%. It is concluded that the foreign gene phyA was expressed to a certain extent and that transgenic phytase promoted decomposing of phytic acid of seeds, which led to improvement of inorganic phosphorus content in transgenic seeds.

Link: http://en.scientificcommons.org/38867780