Cloning and Functional Identification of Two MYB Transcription Factors GmMYBJ6 and GmMYBJ7 in Soybean

Journal: ACTA AGRONO MICA SINICA. 2008,34:1179-1187

Author: DU Hai, TANG Xiao-Feng, LIU Lei, YANG Wen-Jie, WU Yan-Min, HUANG Yu-Bi1, and TANG Yi-Xiong

Abstract:

Plant R2R3-myb genes are widely distributed in higher plants and comprise one of the largest known families of regulatory proteins. On the basis of their conserved DNA-binding domain, 2MYB-like genes, designated GmMYBJ6 and GmMYBJ7 (GenBank accession No. DQ902863 and DQ902864), were cloned from soybean (Glycine max L.) cultivar Jilin 3 using reverse transcription PCR (RT-PCR) and rapid application of cDNA ends (RACE) methods. These genes contained an open reading frame (ORF) of 786 and 969 bp in length encoding 261 and 322 amino acids, respectively. Genomic DNA sequence analysis showed that both genes contained 2 introns. The 2 genes expressed in roots, stems, leaves, flowers, and immature seeds of soybean through semiquantitative RT-PCR analysis, and 2MYB proteins had transcriptional activation functions detected by yeast one-hybrid assay. However, the activities of transcription were lower when the introns existed. Fluorescence real-time quantification PCR analysis indicated that the expression of GmMYBJ6 and GmMYBJ7 genes were induced by abscisic acid (ABA) and naphthalene acetic acid (NAA), and their expressions were changed with the inducible time. It is concluded that GmMYBJ6 and GmMYBJ7 genes isolated from soybean are new members of MYBtranscriptionfactor family, which may play key roles in the signal transduction pathways related to ABA and NAA in soybean.

Link: http://www.sciencedirect.com/science/article/pii/S1875278008600425