Seclection of Maize Inbred Lines with Regeneration and Susceptibility to Agrobacterium tumifacien

Journal: Journal of Genetics and Genomics. 2007,34: 749-755

Author: Wang Yu, Shaohong Fu, Ying Wen, Zhiming Zhang, Yanli Xia, Yuzhen Liu, Tingzhao Rong, Guangtang Pan

Abstract:

Ten-maizeinbredlines of maize (Zea mays L.) with high-induction rate and proliferation ability of embryonic calli were selected from 70-maizeinbredlines by immature embryo culturing. Some of the embryonic calli were transferred onto regeneration medium to examine the ability of regeneration, some were transformed via Agrobacterium tumifaciens C58 carrying intron-β-glucuronidase (gus) gene, and GV3301 carrying the green fluorescent protein (gfp) gene to study the susceptibility of different genotypes in maize to A. tumifaciens. All embryonic calli initiated from 10-maizeinbredlines were able to regenerate into plantlets, and the regeneration frequencies of inbredlines 6010, 6038, 6015, 6051, and 6060 were 61.11%, 31.94%, 45%, 33.33%, and 56.94%, respectively, which were higher than that of other lines. Analysis of variance indicated that the susceptibility of the various genotypes in maize to A. tumifacien C58 showed a significant difference among each other, and the inbredlines 6010, 6015, 6051, 6050, 6058, 6060, 6069, 6077 were susceptible to A. tumifacien C58, of which frequency of gus expression were over 70%. Expression of GFP was observed in six-inbredlines (6050, 6015, 6051, 6058, 6069, 6077). The inbredlines 6051, 6010, 6015, 6060, and 6050 had the high regeneration and the susceptibility to A. tumifaciens C58; and the inbredlines 6051, 6015, and 6060 had the high regeneration and the susceptibility to Agrobacterium tumifaciens GV3301.

Link: http://www.sciencedirect.com/science/article/pii/S1673852707600848