Genome-Wide Identification and Expression Analyses of AnSnRK2 gene family under Osmotic Stress in Ammopiptanthus nanus

Yueming Tang, Fengzhong Lu, Wenqi Feng, Yuan Liu, YangCao, Wanchen Li, Fengling Fu, Haoqiang Yu

Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Maize Research Institute,

Sichuan Agricultural University, Chengdu 611130, China;

Correspondence: ffl@sicau.edu.cn (F.F.); yhq1801@sicau.edu.cn (H.Y.)

Doi:10.3390/plants10050882

Abstract:Sucrose non-fermenting-1 (SNF1)-related protein kinase 2’s (SnRK2s) are plant-specific serine/threonine protein kinases and play crucial roles in the abscisic acid signaling pathway and abiotic stress response. Ammopiptanthus nanus is a relict xerophyte shrub and extremely tolerant of abiotic stresses. Therefore, we performed genome-wide identification of the AnSnRK2 genes and analyzed their expression profiles under osmotic stresses including drought and salinity. A total of 11 AnSnRK2 genes (AnSnRK2.1-AnSnRK2.11) were identified in the A. nanus genome and were divided into three groups according to the phylogenetic tree. The AnSnRK2.6 has seven introns and others have eight introns. All of the AnSnRK2 proteins are highly conserved at the N-terminus and contain similar motif composition. The result of cis-acting element analysis showed that there were abundant hormone- and stress-related cis-elements in the promoter regions of AnSnRK2s. Moreover, the results of quantitative real-time PCR exhibited that the expression of most AnSnRK2s was induced by NaCl and PEG-6000 treatments, but the expression of AnSnRK2.3 and AnSnRK2.6 was inhibited, suggesting that the AnSnRK2s might play key roles in stress tolerance. The study provides insights into understanding the function of AnSnRK2s.

Keywords:protein kinase; SnRK2; osmotic stress; expression profiles; Ammopiptanthus nanus